ASCP Histotechnician Certification Practice Exam 2025 – Your All-in-One Guide to Exam Success!

The light microscope is primarily used in histology because it allows for the examination of tissue samples at a cellular level using visible light. This type of microscope is essential in histology for the preparation and staining of tissue sections, enabling the visualization of the morphology and arrangement of cells within the tissues.

Histologists frequently utilize light microscopy for routine diagnostics, given that it provides adequate resolution to observe cellular details, including nuclei and cytoplasmic structures after samples have been stained with appropriate histological dyes. The techniques involved—such as paraffin embedding, sectioning, and staining—are well adapted for use with light microscopes, making them a staple in pathology labs.

Other microscope types, such as the scanning electron microscope and transmission electron microscope, are generally employed when higher magnification and resolution are needed to view detailed ultrastructure or surface topology, which is more specific to electron microscopy rather than routine histological analysis. The atomic force microscope, while valuable for surface characterization at the nanoscale, is not suitable for traditional tissue examination because it does not allow for the visualizing of biological structures in the same way as light microscopy does.